Affinity ITC

Affinity ITC

The Affinity ITC provides unmatched sample reproducibility with the highest sensitivity, innovative technology, and industry-proven reliability.

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The Affinity ITC and ITC Auto are designed for the most challenging life science laboratory environments that require high sensitivity, high productivity and the most advanced ITC technologies. The Affinity ITC brings advanced engineering to all critical aspects of the measurement ensuring the highest quality ITC data.

Features:

AccuShot delivers the titrant to the right location for the best mixing
FlexSpin provides innovative slow speed stirring, efficient mixing and highest sensitivity
Fully automated, user-selectable system cleaning routines eliminate run-to-run contamination
Intelligent Hardware Positioning for precise reliable injections
Solid-state active heating and cooling for true isothermal temperature control
Choice of standard volume (1.0 mL) or low volume cells (190 µL)
Industry proven 96-well, temperature-controlled liquid handling autosampler. Autosampler can be included with initial purchase or added at a later date
Powerful ITCRun and NanoAnalyze for the most comprehensive suite of tools for method optimization, model fitting, batch analysis, graphing and data export.

TA Instruments has perfected what others have attempted. The Affinity ITC is a powerful tool for measuring a wide variety of molecular interactions. It provides both inexperienced and advanced ITC users the highest confidence in generating superior ITC data.

Affinity ITC and ITC Auto Specifications	Standard Volume	Low Volume
Cell Geometry	Fixed Cylindrical	Fixed Cylindrical
Cell Composition	24K Gold / Hastelloy	24K Gold
Active Cell Volume	1.0 mL	190 µL
Operating Temperature Range	2 °C to 80 °C	2 °C to 80 °C
Minimum Detectable Heat	0.1 µJ	0.05 µJ
Maximum Measureable Heat	5,000 µJ	5,000 µJ
Low Noise Level	0.0025 µW	0.0014 µW
Baseline Stability	0.02 µW/hr	0.02 µW/hr
Temperature Stability	0.00005 °C at 25 °C	0.00005 °C at 25 °C
Temperature Control	Active heating & cooling	Active heating & cooling
Response Time	13 sec / 18 sec	11 sec
Injection Syringe Volume	up to 250 µL	up to 250 µL
Injection Volume Precision	0.01 µL	0.01 µL
Stirring Speed Range	0 to 200 rpm	0 to 200 rpm
Injection Syringe Cleaning/Rinsing	Fully automated	Fully automated
Wash/Rinse Solvent Ports	4 on back of instrument	4 on back of instrument
Automation Specifications
Full Automation Available	24K Gold or Hastelloy	24K Gold
Autosampler Tray Temperature Range	Ambient to 4 °C	Ambient to 4 °C
Sample Capacity	96 (96-well plate format)	96 (96-well plate format)
Wash/Rinse Solvent Ports	5 on side of autosampler	5 on side of autosampler

Optimized Cell Shape

Nano ITC Optimized Cell Shape

Optimized cell shape, material, and volume for greatest accuracy over widest range of sample chemistries.

Solid-State Thermoelectric Elements

Nano ITC Solid-State Thermoelectric Elements

Solid-state thermoelectric elements for active heating and cooling of the sample and reference cells

FlexSpinTM

FlexSpin

Nano ITC New FlexSpin technology dramatically improves mixing, one of the most important aspects of ITC experiments.

AccuShot

AccuShot

Nano ITC AccuShot delivers the right amount of titrant in the right location, every time ensuring the highest quality ITC data.

Industry-Proven Automated Liquid Handling System

Industry-Proven Automated Liquid Handling System

Nano ITC Most dependable automated liquid handling system features an HPLC grade autosampler with temperature controlled storage of a 96-well sample plate.

Intelligent Hardware Positioning

Nano ITC Intelligent Hardware Positioning

Intelligent Hardware Positioning eliminates misalignment, broken and bent syringes and reusability issues seen in previous attempts of ITC automation.

Automated Cleaning System

Automated Cleaning System

Nano ITC User programmable 5 solvent ports allow thorough cleaning of the complete sample path between titrations, eliminating any potential run-to-run cross contamination.

ITC Auto Reproducibility

ITC AUTO Reproducibility

Nano ITC

The Affinity ITC Auto provides unmatched sample reproducibility with the highest sensitivity and reliability. The figure shows twelve titrations performed with complete system cleaning performed between each titration. Data plots are offset for display purposes.

ITC Auto Cleaning Efficiency

ITC AUTO Cleaning Efficiency

Complete system cleaning is user-programmable with Affinity ITC Auto instrument control software. Choosing from five (5) solvent ports ensures the entire sample path is clean. The buffer titrations before and after the protein-ligand titrations provide the highest confidence in the cleaning protocol for the Affinity ITC Auto.

Affinity ITC Titration: Cu – BSA

AFFINITY ITC Titration: Cu – BSA

Nano ITC

Copper (CU) titration into Bovine Serum Albumin (BSA): An example of unmatched data quality obtained on the Affinity ITC. Only the Affinity ITC produces useable first injections with stirring and injection technology that overcomes excess titrant diffusion and produces the most rapid return to baseline between injections.